My research focuses on optimising the analysis of protein species by ESI compatible surface sampling techniques such as LESA (Advion) and the flowprobe (Prosolia). Optimisation of extraction, separation and then structural characterisation of protein (and other biologically relevant species) from biological substrates such as dried blood spots and thin tissue sections is a particular focus. Parameters such as extraction solvent and sampling protocol have been optimised by each sampling procedure. Separation of molecular species extracted from biological smples has been achieved by coupling to an ultra FAIMS chip device (Owlstone, UK). Detected species have been identified via tandem MS/MS experiments e.g. CID, HCD and ETD. Data has been acquired using the following instrumentation: LTQ Orbitrap Velos/Elite (Thermo Fisher Scientific), Synapt G2S (Waters).
- 11) VA Mikhailov, RL Griffiths, HJ Cooper. Liquid Extraction Surface Analysis for Native Mass Spectrometry: Protein Complexes and Ligand Binding. Analytical Chemistry, in press.
- 10) RL Griffiths, AJ Creese, AM Race, J Bunch, HJ Cooper. LESA FAIMS mass spectrometry for the spatial profiling of proteins from tissue. Analytical Chemistry, 6758-6766.
- 9) RL Griffiths, HJ Cooper. Direct Tissue Profiling of Protein Complexes: Toward Native Mass Spectrometry Imaging, Analytical Chemistry, 606-609.
- 8) RL Griffiths, A Dexter, AJ Creese, HJ Cooper. Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots. Analyst, 6879-6885.
- 7) J Sarsby, RL Griffiths, AM Race, J Bunch, EC Randall, AJ Creese, HJ Cooper. Liquid extraction surface analysis mass spectrometry coupled with field asymmetric waveform ion mobility spectrometry for analysis of intact proteins from biological substrates. Analytical Chemistry, 6794−6800.
- 6) NJ Martin, RL Griffiths, RL Edwards, HJ Cooper. Native Liquid Extraction Surface Analysis Mass Spectrometry: Analysis of Noncovalent Protein Complexes Directly from Dried Substrates. Journal of the American Society for Mass Spectrometry, 1152-1158.
- 5) RL Griffiths, J Sarsby, EJ Guggenheim, AM Race, RT Steven, J Fear PF Lalor, J Bunch. Formal Lithium Fixation Improves Direct Analysis of Lipids in Tissue by Mass Spectrometry. Analytical Chemistry, Page 7146–7153.
- 4) R Singal, JB Carrigan, W Wei, P Taniere, RK Hejmadi, C Forde, C Ludwig, J Bunch, RL Griffiths, PJ Johnson, O Tucker, D Alderson, UL Gunther, DG Ward. MALDI profiles of proteins and lipids for the rapid characterisation of upper GI-tract cancers. Journal of Proteomics, Page 207-215.
- 3) JM Fonville, CL Carter, L Pizarro, RT Steven, AD Palmer, RL Griffiths, PF Lalor, JC Lindon, JK Nicholson, E Holmes, J Bunch. Hyperspectral Visualization of Mass Spectrometry Imaging Data. Analytical Chemistry, Page 1415-1423.
- 2) RL Griffiths, J Bunch. A survey of useful salt additives in matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry of lipids: introducing nitrates for improved analysis. Rapid communicatioms in mass spectrometry, Page 1557-1566.
- 1) AD Palmer, RL Griffiths, I Styles, E Claridge, A Calcagni, J Bunch. Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry. Journal of mass spectrometry, Page 237-241.