Welcome to the Cooper mass spectrometry group
Our research focuses on the development of novel methods for
in situ analysis of biomolecules by mass spectrometry.
Liquid extraction surface analysis (LESA) mass spectrometry
We are developing methods for direct sampling of surfaces (dried blood spots, tissue sections, membranes) coupled with high resolution mass spectrometry for targeted and untargeted analysis of proteins and small molecules. For example, we have developed a fast, unambiguous method for diagnosis of haemoglobin variants in newborns. We are currently developing native LESA mass spectrometry.
High field asymmetric waveform ion mobility spectrometry (FAIMS), also known as differential ion mobility offers advantages for the analysis of biomolecules. In addition to unravelling the underlying mechanisms of FAIMS, we are developing methods to couple FAIMS with LESA mass spectrometry and with proteomics for improved analysis of proteins. We are also developing software for interpretation and visualisation of FAIMS data.
Electron mediated dissociation
We are interested in understanding the fundamentals of gas-phase peptide fragmentation, specifically electron capture dissociation, electron transfer dissociation and related approaches, by use of a model peptide approach. We are also developing electron mediated approaches for targeted analysis of protein post-translational modifications.