Dr Oliver Hale

Oliver Hale, mass spectrometry postdoc

I joined the Cooper group as a research fellow in April 2019 to conduct research for the native ambient mass spectrometry (NAMS) project (EPSRC ref: EP/S002979/1). In this project, we aim to apply a variety of sampling techniques for analysing proteins under physiological conditions. For example, using mass spectrometry imaging systems to spatially resolve proteins and protein complexes in body tissues, while retaining information about their 3D structure. I have a research background in analytical chemistry, including high performance liquid chromatography (HPLC), matrix-assisted laser desorption/ionisation mass spectrometry (MALDI MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). I was awarded my PhD from the University of Reading in 2018, after completing a research project focused on the development of atmospheric pressure (AP)-MALDI coupled to ion mobility spectrometry-mass spectrometry for the analysis of biological liquid samples.

Publications

2021

  1. O.J. Hale, J. W. Hughes and H.J. Cooper. Simultaneous spatial, conformational, and mass analysis of intact proteins and protein assemblies by nano-DESI travelling wave ion mobility mass spectrometry imaging, International Journal of Mass Spectrometry, 2021, 468, 116656.
  2. O.J. Hale and H.J. Cooper. Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI, Analytical Chemistry, 2021, 91 (22), 14192-14197.
  3. 2020

    1. O.J. Hale and H.J. Cooper. Native Mass Spectrometry Imaging and In Situ Top-Down Identification of Intact Proteins Directly from Tissue Journal of the American Society for Mass Spectrometry, 31, 12, 2531–2537.
    2. O.J. Hale, E. Illes-Toth, T.H. Mize, and H.J. Cooper. High-Field Asymmetric Waveform Ion Mobility Spectrometry and Native Mass Spectrometry: Analysis of Intact Protein Assemblies and Protein Complexes. Analytical Chemistry, 92, 10, 6811-6816.
    3. O.J. Hale, E.K. Sisley, R.L. Griffiths, I.B. Styles and H.J. Cooper. Native LESA TWIMS-MSI: spatial, conformational and mass analysis of proteins and protein complexes. Journal of the American Society for Mass Spectrometry, 31, 4, 873-879.
    4. O.J. Hale and H.J. Cooper. In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates. Biochemical Society Transactions, BST20190793.

    2019

    1. O.J. Hale and R. Cramer. Atmospheric Pressure Ultraviolet Laser Desorption and Ionization from Liquid Samples for Native Mass Spectrometry. Analytical Chemistry, 14192-14197.
    2. O.J. Hale, M. Morris, B. Jones, C.K. Reynolds and R. Cramer. Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Adds Enhanced Functionalities to MALDI MS Profiling for Disease Diagnostics. ACS Omega, 4, 7, 12759-12765.

    2018

    1. O.J. Hale, P. Ryumin, J.M. Brown, M. Morris and R. Cramer. Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 1-8.
    2. O.J. Hale and R. Cramer. Collision-induced dissociation of doubly-charged barium-cationized lipids generated from liquid samples by atmospheric pressure matrix-assisted laser desorption/ionization provides structurally diagnostic product ions. Analytical and Bioanalytical Chemistry, 1435-1444.

    2016

    1. J.F. Timms, O.J. Hale and R. Crammer. Advances in mass spectrometry-based cancer research and analysis: from cancer proteomics to clinical diagnostics. Expert Review of Proteomics, 593-607.