James Hughes

James Hughes, mass spectrometry PhD student

I am a Sci-Phy student working to develop liquid extraction surface analysis (LESA) mass spectrometry for the detection and spatial profiling of fatty acid binding protein 1 (FABP1) and its variant (FABP1TA) within liver tissues. Computational tools will be developed to allow data fusion and permit quantitation of relative and absolute abundance of biomarkers within tissue. The underlying hypothesis is that levels of FABP1 and FABP1TA, their spatial location, and relative concentrations are indicators of non-alcoholic fatty liver disease (NAFLD) progression. The long term goal is to correlate protein ratios to measurements in serum for diagnostic purposes.



  1. O.J. Hale, J. W. Hughes and H.J. Cooper. Simultaneous spatial, conformational, and mass analysis of intact proteins and protein assemblies by nano-DESI travelling wave ion mobility mass spectrometry imaging, International Journal of Mass Spectrometry, 2021, 468, 116656.
  2. 2020

    1. R.L. Griffiths, J. Hughes, S.E. Abbatiello, M.W. Belford, I.B. Styles and H.J. Cooper. Comprehensive LESA Mass Spectrometry Imaging of Intact Proteins by Integration of Cylindrical FAIMS. Analytical Chemistry, 2885-2890.


    1. D. Lighter, J. Hughes, I.B. Styles, A. Filer and H. Dehghani. Multispectral, non-contact diffuse optical tomography of healthy human finger joints. Biomedical Optics Express, 1445-1460.