Dr Klaudia Kocurek

Klaudia Kocurek, mass spectrometry PhD student

My project involves applying liquid surface extraction analysis (LESA) coupled with mass spectrometry (and aided by high-field asymmetric waveform ion mobility separation, FAIMS) to bacterial colonies growing on solid media. Initially, the focus will be put on verifying the consistency and reproducibility of results across a range of growth and storage conditions. Further testing will aim towards characterising the effects of the extraction process itself when applied to the surfaces and interiors of microbial colonies, as well as exploring the technique's potential as a diagnostic tool for the rapid identification of microbes directly on culture plates with no additional sample preparation.



  1. K.I. Kocurek, J. Havlikova, E. Buchan, A. Tanner, R.C. May and H.J. Cooper. Electroporation and Mass Spectrometry: A New Paradigm for In Situ Analysis of Intact Proteins Direct from Living Yeast Colonies. Analytical Chemistry.


  1. K.I. Kocurek, R.C. May and H.J. Cooper. Application of High-Field Asymmetric Waveform Ion Mobility Separation to LESA Mass Spectrometry of Bacteria. Analytical Chemistry, 4755-4761.


  1. K.I. Kocurek, R.L. Griffiths and H.J. Cooper. Ambient ionization mass spectrometry for in situ analysis of intact proteins. Journal of Mass Spectrometry, 565-578.


  1. R.L. Griffiths, K.I. Kocurek and H.J. Cooper. Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins. Current Opinion in Chemical Biology, 67-75.
  2. K.I. Kocurek, L. Stones, J. Bunch, R.C. May and H.J. Cooper. Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria. Journal of the American Society for Mass Spectrometry, 1-12.