Jana Havlikova

Jana Havlikova, mass spectrometry PhD student

I am a PhD student from Sci-Phy Centre for Doctoral Training and my project is focused on the characterization and classification of microbes causing an infection in posttraumatic wounds. The project aims to develop a method for understanding liquid extraction surface analysis mass spectrometry (LESA MS) of microbes while the research will lead (with a long-term view) towards rapid, direct analysis of samples from patients. The LESA MS analysis will be performed on bacteria and fungi growing on substrates with increasing complexity, including the development of a method for characterization of mixed biofilms and in vitro or ex vivo skin models. The project also involves development/improvement of tools for computational analysis of proteins and microbes from LESA MS spectra.

Publications

2021

  1. J. Havlikova, R.C. May, I.B. Styles and H.J. Cooper. Liquid Extraction Surface Analysis Mass Spectrometry of ESKAPE Pathogens. Journal of the American Society for Mass Spectrometry, 2021
  2. 2020

    1. J. Havlikova, R.C. May, I.B. Styles and H.J. Cooper. Direct identification of bacterial and human proteins from infected wounds in living 3D skin models. Scientific Reports, 10, 11900.
    2. K.I. Kocurek, J. Havlikova, E. Buchan, A. Tanner, R.C. May and H.J. Cooper. Electroporation and Mass Spectrometry: A New Paradigm for In Situ Analysis of Intact Proteins Direct from Living Yeast Colonies. Analytical Chemistry, 2605-2611.

    2019

    1. J. Havlikova, E.C. Randall, R.L. Griffiths, J.G. Swales, R.J.A. Goodwin, J. Bunch, I.B. Styles and H.J. Cooper. Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry. Analytical Chemistry, 14198-14202.